Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G19290.1
Common NameABF4, AREB2, BZIP38, MVI11.7
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 431aa    MW: 46492.1 Da    PI: 10.1974
Description ABRE binding factor 4
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G19290.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
       bZIP_1   5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkev 56 
                  +r+rr++kNRe+A rsR+RK+a++ eLe ++++L++ N++L+k+  e+ ++ 
                  69***************************************97655554443 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003387.5E-11349414IPR004827Basic-leucine zipper domain
PROSITE profilePS5021710.726351400IPR004827Basic-leucine zipper domain
PfamPF001705.6E-11353405IPR004827Basic-leucine zipper domain
SuperFamilySSF579595.34E-10353402No hitNo description
CDDcd147075.61E-23353400No hitNo description
Gene3DG3DSA: hitNo description
PROSITE patternPS000360356371IPR004827Basic-leucine zipper domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009414Biological Processresponse to water deprivation
GO:0009651Biological Processresponse to salt stress
GO:0009738Biological Processabscisic acid-activated signaling pathway
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:1900057Biological Processpositive regulation of leaf senescence
GO:1903648Biological Processpositive regulation of chlorophyll catabolic process
GO:0005634Cellular Componentnucleus
GO:0000976Molecular Functiontranscription regulatory region sequence-specific DNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000005anatomycultured plant cell
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 431 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.239930.0flower| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G19290-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, leaves, flowers and immatures siliques. {ECO:0000269|PubMed:11005831, ECO:0000269|PubMed:11884679}.
Functional Description ? help Back to Top
Source Description
TAIRbZIP transcription factor with specificity for abscisic acid-responsive elements (ABRE). Mediate ABA-dependent stress responses.
UniProtFunctions as transcriptional activator in the ABA-inducible expression of rd29B. Binds specifically to the ABA-responsive element (ABRE) of the rd29B gene promoter. {ECO:0000269|PubMed:11005831, ECO:0000269|PubMed:11884679, ECO:0000269|PubMed:15361142, ECO:0000269|PubMed:16463099}.
Function -- GeneRIF ? help Back to Top
  1. CPK32 is an ABA signaling component that regulates the ABA-responsive gene expression via ABF4.
    [PMID: 16299177]
  2. AREB1, AREB2, and ABF3 are master transcription factors that regulate the ABRE-dependent expression of water-stress responsive genes in ABA signaling in response to water stresses in a cooperative manner.
    [PMID: 19947981]
  3. Data show that the HD-Zip protein Athb-21 and B-3 AP2/ERF transcription factor ESE1 are interact with the abscisic acid responsive element binding factor AREB2/ABF4, which binds to a G-box absolutely required for expression of the COX5b-1 gene.
    [PMID: 22669746]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated by drought, salt, abscisic acid (ABA) and cold. {ECO:0000269|PubMed:10636868, ECO:0000269|PubMed:11005831, ECO:0000269|PubMed:16284313, ECO:0000269|PubMed:16463099}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT3G24650 (R)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT5G52300(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G19290, AT3G23220, AT4G25480, AT4G34000, AT5G05410, AT1G45249, AT1G49720
IntActSearch Q9M7Q2
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Defective in ABA and stress responses. {ECO:0000269|PubMed:15361142}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G19290
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0171610.0AB017161.1 Arabidopsis thaliana mRNA for ABA-responsive element binding protein 2 (AREB2), complete cds.
GenBankAF0935470.0AF093547.1 Arabidopsis thaliana clone 19 abscisic acid responsive elements-binding factor (ABRE) mRNA, partial cds.
GenBankAY0508970.0AY050897.1 Arabidopsis thaliana putative abscisic acid responsive elements-binding factor (At3g19290) mRNA, complete cds.
GenBankAY0912980.0AY091298.1 Arabidopsis thaliana putative abscisic acid responsive elements-binding factor (At3g19290) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_566629.10.0ABRE binding factor 4
SwissprotQ9M7Q20.0AI5L7_ARATH; ABSCISIC ACID-INSENSITIVE 5-like protein 7
TrEMBLF4JB530.0F4JB53_ARATH; ABRE binding factor 4
STRINGAT3G19290.30.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Choi H,Hong J,Ha J,Kang J,Kim SY
    ABFs, a family of ABA-responsive element binding factors.
    J. Biol. Chem., 2000. 275(3): p. 1723-30
  2. Finkelstein RR,Lynch TJ
    The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor.
    Plant Cell, 2000. 12(4): p. 599-609
  3. Uno Y, et al.
    Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions.
    Proc. Natl. Acad. Sci. U.S.A., 2000. 97(21): p. 11632-7
  4. Riechmann JL,Ratcliffe OJ
    A genomic perspective on plant transcription factors.
    Curr. Opin. Plant Biol., 2000. 3(5): p. 423-34
  5. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  6. Kang JY,Choi HI,Im MY,Kim SY
    Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling.
    Plant Cell, 2002. 14(2): p. 343-57
  7. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
  8. Im YJ,Han O,Chung GC,Cho BH
    Antisense expression of an Arabidopsis omega-3 fatty acid desaturase gene reduces salt/drought tolerance in transgenic tobacco plants.
    Mol. Cells, 2002. 13(2): p. 264-71
  9. Bensmihen S, et al.
    The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis.
    Plant Cell, 2002. 14(6): p. 1391-403
  10. Suzuki M,Ketterling MG,Li QB,McCarty DR
    Viviparous1 alters global gene expression patterns through regulation of abscisic acid signaling.
    Plant Physiol., 2003. 132(3): p. 1664-77
  11. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  12. Kim S,Kang JY,Cho DI,Park JH,Kim SY
    ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance.
    Plant J., 2004. 40(1): p. 75-87
  13. Fujita Y, et al.
    AREB1 Is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis.
    Plant Cell, 2005. 17(12): p. 3470-88
  14. Choi HI, et al.
    Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity.
    Plant Physiol., 2005. 139(4): p. 1750-61
  15. Furihata T, et al.
    Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(6): p. 1988-93
  16. Nakashima K, et al.
    Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis.
    Plant Mol. Biol., 2006. 60(1): p. 51-68
  17. Popescu SC, et al.
    Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(11): p. 4730-5
  18. Zhang Y, et al.
    SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis.
    Plant Cell, 2007. 19(6): p. 1912-29
  19. Zhu SY, et al.
    Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis.
    Plant Cell, 2007. 19(10): p. 3019-36
  20. Garcia ME,Lynch T,Peeters J,Snowden C,Finkelstein R
    A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings.
    Plant Mol. Biol., 2008. 67(6): p. 643-58
  21. Comelli RN,Viola IL,Gonzalez DH
    Characterization of promoter elements required for expression and induction by sucrose of the Arabidopsis COX5b-1 nuclear gene, encoding the zinc-binding subunit of cytochrome c oxidase.
    Plant Mol. Biol., 2009. 69(6): p. 729-43
  22. Yoshida T, et al.
    AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation.
    Plant J., 2010. 61(4): p. 672-85
  23. Lee SJ, et al.
    DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity.
    Plant Physiol., 2010. 153(2): p. 716-27
  24. Kriegs B, et al.
    Cyclic monoterpene mediated modulations of Arabidopsis thaliana phenotype: effects on the cytoskeleton and on the expression of selected genes.
    Plant Signal Behav, 2010. 5(7): p. 832-8
  25. Kline KG,Barrett-Wilt GA,Sussman MR
    In planta changes in protein phosphorylation induced by the plant hormone abscisic acid.
    Proc. Natl. Acad. Sci. U.S.A., 2010. 107(36): p. 15986-91
  26. Li G, et al.
    Two cotton Cys2/His2-type zinc-finger proteins, GhDi19-1 and GhDi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling.
    Plant Mol. Biol., 2010. 74(4-5): p. 437-52
  27. Kim YY,Jung KW,Yoo KS,Jeung JU,Shin JS
    A stress-responsive caleosin-like protein, AtCLO4, acts as a negative regulator of ABA responses in Arabidopsis.
    Plant Cell Physiol., 2011. 52(5): p. 874-84
  28. Zhao R, et al.
    The Arabidopsis Ca(2+) -dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth.
    New Phytol., 2011. 192(1): p. 61-73
  29. Rushton DL, et al.
    WRKY transcription factors: key components in abscisic acid signalling.
    Plant Biotechnol. J., 2012. 10(1): p. 2-11
  30. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
  31. Kim JS, et al.
    An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis.
    Plant Cell Physiol., 2011. 52(12): p. 2136-46
  32. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
  33. Zhao R,Wang XF,Zhang DP
    CPK12: A Ca2+-dependent protein kinase balancer in abscisic acid signaling.
    Plant Signal Behav, 2011. 6(11): p. 1687-90
  34. Curran A, et al.
    Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates.
    Front Plant Sci, 2011. 2: p. 36
  35. Comelli RN,Welchen E,Kim HJ,Hong JC,Gonzalez DH
    Delta subclass HD-Zip proteins and a B-3 AP2/ERF transcription factor interact with promoter elements required for expression of the Arabidopsis cytochrome c oxidase 5b-1 gene.
    Plant Mol. Biol., 2012. 80(2): p. 157-67
  36. Luo X, et al.
    Over-expression of GsZFP1, an ABA-responsive C2H2-type zinc finger protein lacking a QALGGH motif, reduces ABA sensitivity and decreases stomata size.
    J. Plant Physiol., 2012. 169(12): p. 1192-202
  37. Lynch T,Erickson BJ,Finkelstein RR
    Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response.
    Plant Mol. Biol., 2012. 80(6): p. 647-58
  38. Luo X, et al.
    Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling.
    J. Exp. Bot., 2013. 64(8): p. 2155-69
  39. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73